Details
SimpliNano is a simple-to-use microvolume spectrophotometer for straightforward concentration and purity measurements of nucleic acids and proteins. SimpliNano uses a built-in sample port that can be used with a variety of common lab chemicals and has no moving parts, making it reliable, easy-to-use, and clean. It has a fixed pathlength that does not require any calibration.
The ability to pipette volumes down to 1 μl directly onto the sample port eliminates the need for cuvettes or other sampling tools. After measurement, the sample can either be easily recovered with a pipette or quickly discarded by wiping the sample port clean for the next measurement.
SimpliNano is preprogrammed with a range of preset methods for the quantitation of nucleic acids and direct UV proteins, with graphical display of resulting wavescans. The SimpliNano direct user interface means that there is no need for a computer, so fast instrument start-up is possible.
| • | Simple sample handling: Built-in sample port with one fixed pathlength means that samples can be added and removed without the need for time-consuming pathlength calibration. |
| • | No moving parts: Contributes to excellent instrument reproducibility and low maintenance. |
| • | Low sample volumes: Reduce sample loss and eliminate the need for dilution by using low volumes of 1 to 5 μl for sample measurement. The sample can either be recovered or simply wiped away after measurement. |
| • | Built-in life science methods: Measures nucleic acid concentrations at 260 nm, protein concentrations at 280 nm, and provides 230/260 and 260/280 ratios for determining sample purity. Wavescan plots from 220 nm to 330 nm can be achieved for nucleic acids and from 250 nm to 350 nm for protein. The methods include options for background correction at 320 nm and 340 nm. |
| • | Flexible instrument control: Use as either a standalone instrument using its large, built-in screen for quick measurements or operate from a PC with optional Datrys software. |
| • | Choice of data output: Output to any suitable PC via a USB cable, to an optional integrated printer, or to a USB stick. |
| Light Source | Xenon |
| Detection Technology | CCD Array |
| Wavelength | 190-1100 nm |
| Absorbance Range | -0.3 to 2.5 Abs. units (10 mm equivalent to 50 Abs. units) |
| Path Length | 0.5 mm |
| Digital Output | To PC via USB cable (standard); USB memory stick (standard); integrated printer (optional) |
| Dimensions | 260 × 390 × 130 mm |
| Weight | Approx. 3 kg |
| Voltage | 90 to 250 V |
| Frequency | 50/60 Hz |
| Current | max 30 VA |
| R-Recommended S-Suitable NS-Not suitable | Ultrospec 2100 | NanoVue Plus | SimpliNano | GeneQuant 1300 |
| DNA/RNA & direct (A280) protein quantitation | R | R | R | R |
| PCR/sequencing/hybridization Primer quantitation and design | S | R | S | R |
| Protein determination (Bradford, Biuret, BCA etc) | R | R | NS | R |
| Cell culture OD600 | R | S | NS | R |
| Enzyme activity kinetics at 340 nm | S | NS | NS | S |
| Fluorescent probe quantitation, cDNA probes for microarrays, PCR probes | S | R | NS | R |
| European pharmacopeia compatibility | NS | NS | NS | NS |
| Pharma method development | NS | NS | NS | NS |