0.5mm

Typically within 2%

Within The DNA, RNA, Oligo and protein A280 methods a continuous spectrum is produced.

The SimpliNano measures 260/280 and A260/A230 ratios for Nucleic Acids and A260/A280 ratios for proteins.

Yes. Absorbance measurements are not specific for a particular nucleic acid and will be affected by the presence of other molecules which absorb at 260 nm.

The sample port is easy to clean. Simply wipe with a lint free tissue or cloth. If a difficult or sticky sample or dye was used, the port can be cleaned with a dilute (2%) detergent solution followed by water or isopropanol.

Yes, with a lint-free tissue or cloth. A sample carryover study using 25 mg/ml BSA with successive alternative additions of water and BSA, was repeated 50 times. The data showed no significant change in absorbance readings throughout the study for the water samples or the BSA samples, indicating that there was no carryover. See SimpliNano Datafile for details. For more difficult, sticky, or strongly coloured samples, the port can be cleaned with a dilute (2%) detergent solution, followed by water or isopropanol.

Most common laboratory solvents, including dilute acids, may be used but they should be immediately wiped off the sample head after measuring. The SimpliNano has been tested with methanol, isopropanol (2-propanol), and acetone. However, these solvents may affect the accuracy of the measurement due to their volatility & are therefore not recommended.

To set the reference, the software will ask for 2 separate samples to ensure the quality of the reference baseline instrument is within calibration.

A pulsed xenon flash lamp.

The lamp should last for several years of use & comes with a 3 year warranty. If it does need changing, this should be done by a service engineer.

The lamp is only on when taking measurements.

No, SimpliNano operates as a stand -alone instrument, although it can be connected to a PC through the USB port to enable connection to a remote printer, LAN or electronic data archive. If operating via a computer is critical, then the SimpliNano can be operated using one of the optional Datrys software modules.

Using the PVC software application, data may be stored either manually or automatically in a variety of data formats which include : as an Excel spreadsheet (Excel must be installed on the PC) , Extensible Meta Format (an EMF) graphics file, a comma delimited (csv) data file, a tab delimited (txt) file, Rich Text Format (RTF) or in native PVC format.

Volumes as low as 1 μl can be used. A sample volume of 2 μl is recommended to minimize pipetting errors affecting results.

The SimpliNano has no moving parts so calibration is not required.

For dsDNA the range is 4.0 ng/µl 2500ng/µl and for BSA 0.12mg/ml 50mg/ml.

Typically ± 2 ng/µl for dsDNA samples < 100 ng/µl and ± 2% for samples > 100 ng/µl. For BSA, typically ± 0.1mg/ml for samples 1-50mg/ml and for > 1mg/ml tolerance of ± 2%.

Specifications for precision and accuracy are below. Absorbance Precision Between 0 & 1A; < 0.005 A or 1% of measurement (whichever is greater) 1 to 2 A: 2% of reading; above 2 A: 5% of reading Absorbance Accuracy ± 1% at 257 nm, Potassium Dichromate solution, 0.7 - 0.8 A.

Ensure sample port area is clean and free of dust and particulate prior to starting work. If in doubt, clean with dilute (2%) detergent solution followed by water or isopropanol.